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Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages
http://hdl.handle.net/10098/00028581
http://hdl.handle.net/10098/0002858161d9611f-4dc7-4c4b-a952-0b6f9369a0bf
名前 / ファイル | ライセンス | アクション |
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bd10125819 (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2021-02-09 | |||||
タイトル | ||||||
タイトル | Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Sendai virus V protein decreases nitric oxide production by inhibiting RIG-I signaling in infected RAW264.7 macrophages | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Sendai virus | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | V protein | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Nitric oxide | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RIG-I | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | TRIM25 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Macrophages | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
資源タイプ | other | |||||
著者 |
Morita, Naoko
× Morita, Naoko× Tanaka, Yukie× Odkhuu, Erdenezaya× Naiki, Yoshikazu× Komatsu, Takayuki× Koide, Naoki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Sendai virus V protein is a known antagonist of RIG-I-like receptors (RLRs) RIG-I and MDA5, which activate transcription factors IRF3, leading to activation of ISGF3 and NF-kB. These transcription factors are known activators of inducible NO synthase (iNOS) and increase the production of nitric oxide (NO). By inhibiting ISGF3 and NF-kB, the V protein acts as an indirect negative regulator of iNOS and NO. Here we report that the V gene knockout Sendai virus [SeV V()] markedly enhanced iNOS expression and subsequent NO production in infected macrophages compared to wild-type SeV. The knockout of RIG-I in cells inhibited SeV V()-induced iNOS expression and subsequent NO production. To understand the underlying mechanism of the V protein-mediated negative regulation of iNOS activation, we transfected HEK293T cells with RIG-I and the RIG-I regulatory protein TRIM25. Our results demonstrated that the V protein inhibited iNOS activation via the RIG-I/TRIM25 pathway. Moreover, the V protein inhibited TRIM25-mediated K63-linked ubiquitination of RIG-I, as well as its CARD-dependent interaction with mitochondrial antiviral signaling (MAVS) molecules. These results suggest that the V protein downregulates iNOS activation and inhibits NO production by preventing the RIG-I-MAVS interaction, possibly through its effect on the ubiquitination status of RIG-I. | |||||
書誌情報 |
en : Microbes and Infection 巻 22, 号 8, p. 322-330, 発行日 2020-02-05 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 12864579 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.micinf.2020.01.005 | |||||
権利 | ||||||
権利情報 | copyright 2020 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AO | |||||
出版タイプResource | http://purl.org/coar/version/c_b1a7d7d4d402bcce | |||||
その他のID | ||||||
TD10125819 |