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The human renin gene contains a direct repeat (DR) motifAGGGGTCAC-AGGGCCA in the proximal region (-259/-245 bp), which contains similar sequence for nuclear receptor superfamily binding core motif, AGGTCA, and is the most similar to COUP-TFII consensus. The DR motif was evaluated as a functional cis-element with renal cortex and chorio-decidual cells by footprint assay, electromobility shift assay (EMSA) and reporter assay. The DR motif site was protected by footprint analysis with a clear hypersensitive and a minor hypersensitive region in good accordance with the DR of the consensus. One of the binding proteins was strongly suspected to be COUP-TFII-consensus-specific by EMSA. The DNA/protein complexes obtained with nuclear extract of renin producing cells could be completely blocked by homologous competitor and strongly blocked by the second-half mutant oligonucleotide of the DR motif but not by the first-half mutant oligonucleotide. Finally, the transcriptional activity of second-half mutant construct is slightly elevated and that first-half mutant construct is significantly stronger by twofold compared with wild type construct in reporter assay. These findings suggest that the DR motif site of the human renin gene functions as a negative regulatory element involved in a twofold repression of transcription and that member(s) of nucleic receptor superfamily bind the site and play important roles in the human renin gene expression with a possibility that one of the binding protein is COUP-TFII.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "John Wiley \u0026 Sons"}]}, "item_10001_relation_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "TD00006323", "subitem_relation_type_select": "NCID"}}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "7302312", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KONOSHITA, Tadashi"}], "nameIdentifiers": [{"nameIdentifier": "59692", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUCHS, Sébastien"}], "nameIdentifiers": [{"nameIdentifier": "59693", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MAKINO, Yasukazu"}], "nameIdentifiers": [{"nameIdentifier": "59694", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WAKAHARA, Shigeyuki"}], "nameIdentifiers": [{"nameIdentifier": "59695", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "MIYAMORI, Isamu"}], "nameIdentifiers": [{"nameIdentifier": "59696", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-08-04"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "A_proximal_PDF_file_original.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "A_proximal_PDF_file_original.pdf", "url": "https://u-fukui.repo.nii.ac.jp/record/20637/files/A_proximal_PDF_file_original.pdf"}, "version_id": "88317213-e6a6-423b-9523-4978614d2f9d"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "renin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "renin-angiotensin system", "subitem_subject_scheme": "Other"}, {"subitem_subject": "COUP-TFII", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ARP-1", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NR2F2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cis-element", "subitem_subject_scheme": "Other"}, {"subitem_subject": "trans-factor", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "other", "resourceuri": "http://purl.org/coar/resource_type/c_1843"}]}, "item_title": "A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene"}]}, "item_type_id": "10001", "owner": "1", "path": ["2393"], "permalink_uri": "http://hdl.handle.net/10098/1816", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-12-17"}, "publish_date": "2008-12-17", "publish_status": "0", "recid": "20637", "relation": {}, "relation_version_is_last": true, "title": ["A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene"], "weko_shared_id": -1}
A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene
http://hdl.handle.net/10098/1816
http://hdl.handle.net/10098/18168ba53560-1a15-4682-a08f-57391258bb63
名前 / ファイル | ライセンス | アクション |
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A_proximal_PDF_file_original.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-12-17 | |||||
タイトル | ||||||
タイトル | A proximal direct repeat motif characterized as a negative regulatory element in the human renin gene | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | renin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | renin-angiotensin system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | COUP-TFII | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ARP-1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | NR2F2 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cis-element | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | trans-factor | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_1843 | |||||
資源タイプ | other | |||||
著者 |
KONOSHITA, Tadashi
× KONOSHITA, Tadashi× FUCHS, Sébastien× MAKINO, Yasukazu× WAKAHARA, Shigeyuki× MIYAMORI, Isamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The regulation of renin gene expression is thought to be fundamental to regulation of the total renin-angiotensin system. The human renin gene contains a direct repeat (DR) motifAGGGGTCAC-AGGGCCA in the proximal region (-259/-245 bp), which contains similar sequence for nuclear receptor superfamily binding core motif, AGGTCA, and is the most similar to COUP-TFII consensus. The DR motif was evaluated as a functional cis-element with renal cortex and chorio-decidual cells by footprint assay, electromobility shift assay (EMSA) and reporter assay. The DR motif site was protected by footprint analysis with a clear hypersensitive and a minor hypersensitive region in good accordance with the DR of the consensus. One of the binding proteins was strongly suspected to be COUP-TFII-consensus-specific by EMSA. The DNA/protein complexes obtained with nuclear extract of renin producing cells could be completely blocked by homologous competitor and strongly blocked by the second-half mutant oligonucleotide of the DR motif but not by the first-half mutant oligonucleotide. Finally, the transcriptional activity of second-half mutant construct is slightly elevated and that first-half mutant construct is significantly stronger by twofold compared with wild type construct in reporter assay. These findings suggest that the DR motif site of the human renin gene functions as a negative regulatory element involved in a twofold repression of transcription and that member(s) of nucleic receptor superfamily bind the site and play important roles in the human renin gene expression with a possibility that one of the binding protein is COUP-TFII. | |||||
書誌情報 |
Journal of cellular biochemistry 巻 102, 号 4, p. 1043-1050, 発行日 2007-11 |
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出版者 | ||||||
出版者 | John Wiley & Sons | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 7302312 | |||||
書誌レコードID | ||||||
識別子タイプ | NCID | |||||
関連識別子 | TD00006323 |